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Assessing the pentose phosphate pathway using [2, 3‐ 13 C 2 ]glucose
Author(s) -
Lee Min Hee,
Malloy Craig R.,
Corbin Ian R.,
Li Junjie,
Jin Eunsook S.
Publication year - 2019
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.4096
Subject(s) - pentose phosphate pathway , pentose , chemistry , radiochemistry , phosphate , biochemistry , glycolysis , metabolism , fermentation
[2,3‐ 13 C 2 ]glucose is a novel and specific tracer of the pentose phosphate pathway (PPP). 13 C NMR analysis of lactate after the administration of [2,3‐ 13 C 2 ]glucose informs PPP activity and glycolysis because [1,2‐ 13 C 2 ]lactate arises from glycolysis and [2,3‐ 13 C 2 ]lactate arises exclusively through the PPP. In rats receiving [2,3‐ 13 C 2 ]glucose, the PPP was more active in the fed versus fasted state in most organs. Both the PPP and glycolysis were substantially increased in hepatoma compared to liver. [2,3‐ 13 C 2 ]glucose and 13 C NMR simplify assessment of the PPP.
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