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Manganese‐enhanced MRI (ME MRI) in evaluation of the auditory pathway in an experimental rat model
Author(s) -
Tang Zuohua,
Wu Lingjie,
Xiao Zebin,
Feng Xiaoyuan,
Sun Xinghuai,
Tang Weijun,
Wang Jie,
Jin Lixin
Publication year - 2017
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.3677
Subject(s) - perilymph , tympanum (architecture) , auditory cortex , magnetic resonance imaging , chemistry , medicine , inner ear , nuclear medicine , anatomy , middle ear , audiology , radiology , myringotomy , tympan
This study aimed to explore the optimal dose and manner of administration for visualization of the auditory pathway on manganese‐enhanced MRI (ME MRI). Twenty‐four healthy male Sprague–Dawley rats were randomly divided into three experimental groups ( n = 8 for Groups A, B and C). The rats in Groups A, B and C were subjected to MnCl 2 injection through the tympanum, inner ear endolymph and perilymph, respectively (0.2 M for four rats and 0.4 M for the others in each group) and observed at 1, 2, 3, 4, 7 and 10 days after the operation with 3.0 T MRI. The signal intensity (SI) and dynamic changes of the auditory pathways at various times, and at two doses through three injection routes, were compared by statistical analysis. Administration of MnCl 2 through the perilymph best showed the complete auditory pathway ( P < 0.01), whereas administration though the tympanum only demonstrated part of the pathway. The SI was highest at 24 h after administration of the tracer and began to decline at 48 h. The SI of the auditory cortex was higher after the injection of 0.4 M MnCl 2 than that of 0.2 M MnCl 2 . ME MRI best demonstrated the whole auditory pathway at 24 h after the injection of 0.4 M MnCl 2 through the perilymph in the rat, which provided an optimal method for the study of ME MRI of the auditory pathway in the animal model.