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Initial evaluation of hepatic T 1 relaxation time as an imaging marker of liver disease associated with autosomal recessive polycystic kidney disease (ARPKD)
Author(s) -
Gao Ying,
Erokwu Bernadette O.,
DeSantis David A.,
Croniger Colleen M.,
Schur Rebecca M.,
Lu Lan,
Mariappuram Jose,
Dell Katherine M.,
Flask Chris A.
Publication year - 2016
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.3442
Subject(s) - autosomal recessive polycystic kidney disease , medicine , pathology , liver disease , congenital hepatic fibrosis , fibrosis , bile duct , kidney , gastroenterology , magnetic resonance imaging , cirrhosis , disease , polycystic kidney disease , radiology , portal hypertension
Autosomal recessive polycystic kidney disease (ARPKD) is a potentially lethal multi‐organ disease affecting both the kidneys and the liver. Unfortunately, there are currently no non‐invasive methods to monitor liver disease progression in ARPKD patients, limiting the study of potential therapeutic interventions. Herein, we perform an initial investigation of T 1 relaxation time as a potential imaging biomarker to quantitatively assess the two primary pathologic hallmarks of ARPKD liver disease: biliary dilatation and periportal fibrosis in the PCK rat model of ARPKD. T 1 relaxation time results were obtained for five PCK rats at 3 months of age using a Look–Locker acquisition on a Bruker BioSpec 7.0 T MRI scanner. Six three‐month‐old Sprague‐Dawley (SD) rats were also scanned as controls. All animals were euthanized after the three‐month scans for histological and biochemical assessments of bile duct dilatation and hepatic fibrosis for comparison. PCK rats exhibited significantly increased liver T 1 values (mean ± standard deviation = 935 ± 39 ms) compared with age‐matched SD control rats (847 ± 26 ms, p = 0.01). One PCK rat exhibited severe cholangitis (mean T 1 = 1413 ms), which occurs periodically in ARPKD patients. The observed increase in the in vivo liver T 1 relaxation time correlated significantly with three histological and biochemical indicators of biliary dilatation and fibrosis: bile duct area percent ( R = 0.85, p = 0.002), periportal fibrosis area percent ( R = 0.82, p = 0.004), and hydroxyproline content ( R = 0.76, p = 0.01). These results suggest that hepatic T 1 relaxation time may provide a sensitive and non‐invasive imaging biomarker to monitor ARPKD liver disease. Copyright © 2015 John Wiley & Sons, Ltd.