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Proton observed phosphorus editing (POPE) for in vivo detection of phospholipid metabolites
Author(s) -
Wijnen Jannie P.,
Klomp Dennis W. J.,
Nabuurs Christine I. H. C.,
Graaf Robin A.,
Kalleveen Irene M. L.,
Kemp Wybe J. M.,
Luijten Peter R.,
Kruit Mark C.,
Webb Andrew,
Kan Hermien E.,
Boer Vincent O.
Publication year - 2016
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.3440
Subject(s) - in vivo , phospholipid , proton , chemistry , nuclear magnetic resonance , relaxation (psychology) , phosphocholine , analytical chemistry (journal) , chromatography , phosphatidylcholine , biochemistry , physics , membrane , biology , microbiology and biotechnology , quantum mechanics , neuroscience
The purpose of this article was to compare the sensitivity of proton observed phosphorus editing (POPE) with direct 31 P MRS with Ernst angle excitation for 1 H– 31 P coupled metabolites at 7 T. POPE sequences were developed for detecting phosphocholine (PC), phosphoethanolamine (PE), glycerophosphocholine (GPC), and glycerophosphoethanolamine (GPE) on the 1 H channel, thereby using the enhanced sensitivity of the 1 H nuclei over 31 P detection. Five healthy volunteers were examined with POPE and 31 P‐MRS. POPE editing showed a more than doubled sensitivity in an ideal phantom experiment as compared with direct 31 P MRS with Ernst angle excitation. In vivo , despite increased relaxation losses, significant gains in signal‐to‐noise ratio (SNR) of 30–40% were shown for PE and GPE + PC levels in the human brain. The SNR of GPC was lower in the POPE measurement compared with the 31 P‐MRS measurement. Furthermore, selective narrowband editing on the 31 P channel showed the ability to separate the overlapping GPE and PE signals in the 1 H spectrum. POPE can be used for enhanced detection of 1 H– 31 P coupled metabolites in vivo . Copyright © 2015 John Wiley & Sons, Ltd.