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Effects of exercise‐induced intracellular acidosis on the phosphocreatine recovery kinetics: a 31 P MRS study in three muscle groups in humans
Author(s) -
Layec Gwenael,
Malucelli Emil,
Le Fur Y.,
Manners David,
Yashiro Kazuya,
Testa Claudia,
Cozzone Patrick J.,
Iotti Stefano,
Bendahan David
Publication year - 2013
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.2966
Subject(s) - phosphocreatine , oxidative phosphorylation , skeletal muscle , medicine , chemistry , endocrinology , kinetics , acidosis , intracellular ph , fast twitch muscle , intracellular , biochemistry , energy metabolism , physics , quantum mechanics
Little is known about the metabolic differences that exist among different muscle groups within the same subjects. Therefore, we used 31 P‐magnetic resonance spectroscopy ( 31 P‐MRS) to investigate muscle oxidative capacity and the potential effects of pH on PCr recovery kinetics between muscles of different phenotypes (quadriceps (Q), finger (FF) and plantar flexors (PF)) in the same cohort of 16 untrained adults. The estimated muscle oxidative capacity was lower in Q (29 ± 12 mM min ‐1 , CV inter‐subject = 42%) as compared with PF (46 ± 20 mM min ‐1 , CV inter‐subject = 44%) and tended to be higher in FF (43 ± 35 mM min ‐1 , CV inter‐subject = 80%). The coefficient of variation (CV) of oxidative capacity between muscles within the group was 59 ± 24%. PCr recovery time constant was correlated with end‐exercise pH in Q ( p < 0.01), FF ( p < 0.05) and PF ( p <0.05) as well as proton efflux rate in FF ( p < 0.01), PF ( p < 0.01) and Q ( p = 0.12). We also observed a steeper slope of the relationship between end‐exercise acidosis and PCr recovery kinetics in FF compared with either PF or Q muscles. Overall, this study supports the concept of skeletal muscle heterogeneity by revealing a comparable inter‐ and intra‐individual variability in oxidative capacity across three skeletal muscles in untrained individuals. These findings also indicate that the sensitivity of mitochondrial respiration to the inhibition associated with cytosolic acidosis is greater in the finger flexor muscles compared with locomotor muscles, which might be related to differences in permeability in the mitochondrial membrane and, to some extent, to proton efflux rates. Copyright © 2013 John Wiley & Sons, Ltd.