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Metabolism of hyperpolarized [1‐ 13 C]pyruvate in the isolated perfused rat lung – an ischemia study
Author(s) -
Pullinger B.,
Profka H.,
ArdenkjaerLarsen J. H.,
Kuzma N. N.,
Kadlecek S.,
Rizi R. R.
Publication year - 2012
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.2777
Subject(s) - ischemia , perfusion , lung , chemistry , metabolism , medicine , endocrinology
We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1‐ 13 C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several‐fold increase in lactate labeling was observed immediately after a 25‐min ischemia event as was its reversal back to the baseline after 30–40 min of resumed perfusion ( n = 5, p < 0.025 for both comparisons). These results were corroborated by 31 P spectroscopy and correspond well to measured changes in lactate pool size determined by 1 H spectroscopy of freeze‐clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.