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31 P NMR in vivo study of rat brain energy metabolism after frontal cortex injury. a method based on convoluted correlation matrices of the spectral data
Author(s) -
Semenova Nataly A.,
Konradov Alexander A.,
Dubinsky Vladimir Z.
Publication year - 1995
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940080405
Subject(s) - phosphocreatine , phosphomonoesters , in vivo , energy metabolism , energy charge , bioenergetics , medicine , pi , chemistry , perfusion , endocrinology , biology , biochemistry , enzyme , microbiology and biotechnology , adenylate kinase , mitochondrion
Disturbances of energy metabolism in rat brain after frontal lobectomy and post‐traumatic effects of monosialic ganglioside GM 1 on brain energy metabolism were studied by 31 P NMR in vivo . Frontal lobectomy caused the statistically significant reversible decrease of ATP and increase of ADP during the compensatory period. In a presence of GM, these post‐operational effects were not observed. The mean value of normalized correlation coefficients between NMR‐measured phosphomonoesters, P i , pH, phosphocreatine, γ‐ATP +β‐ADP, α‐ATP+α‐ADP + NAD/NADH + diphosphodiesters and β‐ATP for a given group of animals (Z‐index) was used to estimate brain energetic status. It has been shown that the variation of the Z‐index correlates with the dynamics of locomotor activity during the compensatory period. The Z‐index increased by more than 100% for the subgroup with an increased moving activity and only by about 20% for the subgroup with a decreased activity. Moreover, the compositions of these subgroups were almost the same as those from the solution of the reverse task, i.e., the studied group was divided into two subgroups by the Z‐index. The Z‐index is proposed as a possible criterion of pathology detection in brain energetics.