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Inhibition of aldose reductase: 13 C NMR studies in isolated peripheral nerve
Author(s) -
Waterton John C.,
Breen Seáan A.,
Mirrlees Don J.,
Sennitt Christopher M.,
Carey Frank
Publication year - 1995
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940080309
Subject(s) - aldose reductase , aldose reductase inhibitor , chemistry , flux (metallurgy) , substrate (aquarium) , aldose , galactose , sciatic nerve , extracellular , reductase , biochemistry , stereochemistry , enzyme , anatomy , biology , glycoside , organic chemistry , ecology
Abstract We report 13 C NMR measurements of the flux through aldose reductase in isolated rat sciatic nerve, and its inhibition by an aldose reductase inhibitor of the sulphonylnitromethane class. [1‐ 13 C] galactose was used as substrate, and the rate of production of [1‐ 13 C] dulcitol was measured. Quantitation required the use both of internal extracellular, and external, standards. The mean net forward flux (±S.D) was 20±11 nmol/(mL nerve water)/min (n = 10). In the presence of the inhibitor, flux was reduced significantly (p<0.001) to 13% of control. Since dulcitol is symmetrical, an estimate of the backward flux, to [6‐ 13 C] galactose, is also possible; under our conditions, this was negligible.