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Experimental allergic encephalomyelitis in non‐human primates: mri and mrs may predict the type of brain damage
Author(s) -
Richards T. L.,
Petersen K.,
Heide A. C.,
Cluff J.,
Alvord E. C.,
Rose L. M.,
Peterson J.,
Cosgrove S.,
Petersen R.
Publication year - 1995
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940080202
Subject(s) - encephalomyelitis , creatine , lesion , pathology , choline , myelin , medicine , human brain , magnetic resonance imaging , sensitization , central nervous system , nuclear medicine , radiology , immunology , psychiatry
Volume‐localized proton spectroscopy and T 2 ‐weighted MRI were performed on 23 monkeys with experimental allergic encephalomyelitis (EAE). The purpose of this study was to determine the relationships between temporal changes in lesion activity (measured on T 2 ‐weighted MRI), MRS [N‐acetyl aspartate (NAA), creatine (CR), choline (CHO)], and the histologic definition of disease determined post‐mortem. Animals were scanned in the same areas of the brain once a week before and after sensitization to myelin basic protein (BP). Histologic lesion types were predicted by a combination of preceding MRI and MRS measurements. Acutely fatal EAE lesions were large and monophasic as visualized by MRI, and increased CHO (p<0.02, n=16) and CHO/CR ratio (p<0.001, n = 16) were detected by MRS at disease onset. Chronic EAE lesions were preceded by multiple inflammatory attacks as visualized by MRI and consistently low levels of NAA (p<0.02, n = 13) and NAA/CR (p<0.01, n = 13) which occurred after the initial attack. MRI negative brain regions (from animals that were sensitized to BP) were associated with low CHO/CR (p<0.1, n = 5). The temporal correlation of MRI lesion activity and absolute MRS proton metabolites shows promise for predicting the subsequent duration and histologic type of lesions in EAE in non‐human primates.