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Long‐term observation of in vivo 31 P nmr spectra in carbon tetrachloride‐intoxicated rabbit liver using implanted wireless surface coil
Author(s) -
Morikawa Shigehiro,
Inubushi Toshiro,
Kito Kouichi,
Amano Shigeru
Publication year - 1995
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940080103
Subject(s) - carbon tetrachloride , rabbit (cipher) , nuclear magnetic resonance , spectral line , chemistry , tetrachloride , in vivo , physics , biology , organic chemistry , statistics , mathematics , microbiology and biotechnology , astronomy , tin
Phosphorus‐31 NMR spectra in rabbit liver were successively observed for more than 5 months in a chronic CCI 4 intoxication model using implanted wireless surface coils. The quality of the obtained NMR spectra was satisfactory and there was sufficient time resolution to follow the dynamic changes that occurred during a fructose tolerance test. The implanted coil did not lead to any serious adverse effects such as infection or liver dysfunction. During an 18‐week administration of CCI 4 , the area of the phosphodiester peak significantly decreased. The ratio of P i /β‐ATP was transiently elevated and gradually returned to the control level. In the fructose tolerance test, the increase in phosphomonoester and the decrease in P i and β‐ATP after i.v. fructose loading were apparent, but the degrees in these changes became smaller during the CCI 4 intoxication. This wireless surface coil was useful for consecutive and non‐invasive 31 P NMR observations of the liver to follow chronically treated animal models over a period of several months.