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An experimental approach for dynamic rat liver observation in vivo and ex vivo by 31 P localized MR spectroscopy for follow‐up of liver status at different steps of orthotopic transplantation–a feasibility study
Author(s) -
Volk A.,
Astarcioglu I.,
Adam R.,
Mispelter J.,
Collet V.,
Dimicoli J. L.,
Bismuth H.
Publication year - 1994
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940070807
Subject(s) - ex vivo , in vivo , transplantation , liver transplantation , dephasing , spins , imaging phantom , nuclear magnetic resonance , spectroscopy , coupling (piping) , chemistry , materials science , nuclear medicine , physics , biology , medicine , radiology , microbiology and biotechnology , quantum mechanics , metallurgy
An experimental approach was evaluated to perform a follow‐up of rat liver transplantation by 31 P spectroscopy. The approach is based on the use of an implanted surface coil attached to the liver and inductively coupled to the receiver/transmitter line by an external coupling loop. Spatial localization to the liver was performed by selective excitation and dephasing of spins in a slice between the implanted and the coupling coil, the slice being positioned on a proton image acquired prior to spectroscopy. Characteristics of the protocol were established on a phantom and in vivo on non‐transplanted rat livers indicating good localization and high signal‐to‐noise ratio. Preliminary results obtained on transplanted livers revealed a relation between evolution of the 31 P spectrum and animal survival. As shown in separate experiments, the same technique also enabled easy acquisition of the 31 P profile of livers stored at 4°C in University of Wisconsin solution. Therefore, the experimental approach described here opens up the possibility of measuring changes of the 31 P profile on the same liver during the whole transplantation procedure, i.e., in the donor, during preservation and after transplantation, and comparing evolution of spectra with transplantation outcome.