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13 C NMR kinetic studies of the rapid stimulation of glucose metabolism by estrogen in immature rat uterus
Author(s) -
Shinkarenko Leonid,
Kaye Alvin M.,
Degani Hadassa
Publication year - 1994
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940070503
Subject(s) - glycogen , medicine , endocrinology , stimulation , metabolism , glycolysis , creatine , estrogen , glycogen synthase , chemistry , uterus , glutamate receptor , carbohydrate metabolism , creatine kinase , biochemistry , biology , receptor
Glucose metabolism in control and estrogen stimulated rat uteri was investigated using 13 C NMR spectroscopy. By employing an NMR adapted perifusion system, and developing protocols and methods based on the application of [1‐ 13 C]glucose labeling, it was possible to measure, with a temporal resolution of 10 min, the kinetics of glucose consumption, lactate production and 13 C incorporation into glutamate, alanine and glycogen. In control immature rat uteri, under aerobic conditions, the rates (±SEM) of [1‐ 13 C]glucose consumption and [3‐ 13 C]lactate production were 24±2 and 7.5±0.5mmol/g wet weight/h. The rates of synthesis of [4‐ 13 C]glutamate, [3‐ 13 C]alanine and 13 C labeling of glycogen at C 1 of its glucose moieties were significantly lower and were in the range 0.3–0.6 mmol/g wet weight/h. Thus, ca 35% of the labeled glucose was accounted for by the glycolytic and other observed pathways. In vitro stimulation of the uteri by estrogen was found to increase significantly, within 1 h, glucose consumption by 80%, lactate production by 150% and glutamate and glycogen synthesis by 150%, in parallel to a rapid hormonal induction of mRNA for the brain type isozyme of creatine kinase.