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31 P MRS of tumour metabolism in anaesthetized vs conscious mice
Author(s) -
Sansom J. M.,
Wood P. J.
Publication year - 1994
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940070403
Subject(s) - chloral hydrate , etomidate , chemistry , in vivo , ratón , endocrinology , anesthesia , medicine , biology , microbiology and biotechnology , propofol
The injectable anaesthetics Hypnorm/Hypnovel, chloral hydrate and etomidate, were examined for their effects on C 3 H/He mouse core temperature and on the in vivo 31 P MR spectra of KHT and SCCVII/Ha transplantable tumours, compared with conscious mice gently restrained in jigs. Hypnorm/Hypnovel at 0.1 mL/mouse i.p. reduced core temperature by 6°C at 30 min after injection, returning to control levels by 100 min, but did not significantly alter the 31 P MR spectra of either KHT or SCCVII/Ha tumours. Chloral hydrate at 300 mg/kg i.p. produced a 5°C fall in mouse core temperature, at 25 min after injection, again returning to control levels by 100 min. This agent increased the P i /total ratio to 155% of control at 15 min after injection in the KHT tumour, and to 170% of control at 45 min in SCCVII/Ha. Etomidate at 25 mg/kg i.p. reduced mouse core temperature by 7.5°C by 20 min after injection, returning to only 84% of control by 100 min. This agent increased the P i /total ratio by 260% in the KHT tumour 15 min after injection, without recovery to control values by 100 min. In SCCVII/Ha, a maximum increase in P i /total of 360% was observed at 15 min after injection, with a return to control levels by 60 min. In addition, etomidate caused convulsions in the mice during the induction phase, and myoclonic jerking within 15 min of anaesthesia.