Premium
Non‐invasive investigation of parenchymal liver disease using 31 P NMR spectroscopy
Author(s) -
Roelsgaard Klaus,
StødkildeJørgensen Hans,
Dønstrup Sune,
Djurhuus Jens Christian
Publication year - 1993
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940060606
Subject(s) - nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , spectroscopy , in vivo magnetic resonance spectroscopy , parenchyma , chemistry , pathology , medicine , magnetic resonance imaging , physics , radiology , quantum mechanics
Four 31 P NMR spectroscopy parameters were measured non‐invasively in the liver of 11 healthy pigs and 9 pigs with CCI 4 ‐induced liver disease: (i) absolute molar concentration of phosphorous metabolites; (ii) pH based on the chemical shift of the P i peak; (iii) T 1 of the peaks in the 31 P NMR spectrum; and (iv) changes in ATP, P i and phosphomonoester after fructose administration. Liver disease was verified by histology and blood chemistry. The concentration of ATP decreased from 3.0(2.8–3.1) to 2.0(2.0–2.4) mM (median and quartiles) when liver disease was induced (p<0.05). The concentration of phosphodiesters (PDEs) decreased from 14.8(11.4–19.5) to 8.7(7.4–11.6) mM (p<0.05). pH increased by 0.1 unit. T i relaxation times for the γ‐, α‐ and β‐ATP peaks increased from 320(249–471) to 577(506–638) ms (p<0.01), from 765(611–786) to 906(820–1058) ms (p<0.05) and from 402(327–509) to 579(543–743) ms (p<0.01), respectively, while T 1 for the PDE peak decreased from 2204(1909–2404) to 1758(1502‐1894) ms (p<0.05). In the healthy animals injection of fructose was followed by a reduction of ATP (β‐ATP). In diseased livers this reduction was significantly smaller. In conclusion, it was possible non‐invasively to show differences between healthy and diseased livers in all NMR parameters evaluated. This means that 31 P NMR Spectroscopy may have a potential as a non‐invasive diagnostic method for studying liver disease.