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Quantitative analysis by 31 P magnetic resonance spectroscopy of abnormal mitochondrial oxidation in skeletal muscle during recovery from exercise
Author(s) -
Kemp G. J.,
Taylor D. J.,
Thompson C. H.,
Hands L. J.,
Rajagopalan B.,
Styles P.,
Radda G. K.
Publication year - 1993
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940060504
Subject(s) - anaerobic exercise , phosphocreatine , mitochondrion , medicine , mitochondrial myopathy , oxidative phosphorylation , chemiosmosis , lactic acid , endocrinology , chemistry , atp synthase , skeletal muscle , biochemistry , biology , mitochondrial dna , energy metabolism , physiology , bacteria , enzyme , genetics , gene
We use the hyperbolic relationship between cytosolic [ADP] and the rate of phosphocreatine (PCr) resynthesis after exercise to estimate the apparent maximum rate of oxidative ATP synthesis ( Q MAX ). We examine data from some human diseases in which mitochondrial oxidation may be impaired (due to reduced mitochondrial numbers, intrinsic mitochondrial defect or impaired vascular supply). Muscle responds to impaired oxidation by stimulating anaerobic ATP synthesis and/or by increasing [ADP], the stimulus to the mitochondrion. However, these responses interact: [ADP] depends on pH and [PCr], and lactic acid production tends to lower [ADP] (by lowering pH), while proton efflux has the opposite effect. We identify four patterns of results: (A) in mitochondrial myopathy, apparent Q MAX is reduced and [ADP] is appropriately increased, because increased proton efflux reduces the pH change in exercise despite increased lactic acid production; (B) in some conditions (e.g., cyanotic congenital heart disease) apparent Q MAX is reduced but there is no compensatory rise in [ADP], probably because anaerobic ATP synthesis during exercise is increased without increase in proton efflux; (C) in other conditions (e.g., myotonic dystrophy) [ADP] is increased during exercise but apparent Q MAX is normal, suggesting either an increase in proton efflux and/or decrease in anaerobic ATP synthesis during exercise; (D) there are also conditions (e.g., respiratory failure) where, despite impaired oxygen supply, both apparent Q MAX and end‐exercise [ADP] are normal. We also discuss the metabolic conditions under which end‐exercise [ADP] is increased by a mitochondrial defect. Finally, we discuss some technical aspects, including the effects of an altered mitochondrial K m for ADP and the relationship of the analysis to the halftime of PCr recovery and an alternative calculation of apparent Q MAX .