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Growth and Magnetic Resonance Characteristics of Human Squamous Cell Carcinoma Xenografts Implanted with Cells Suspended in Matrigel TM
Author(s) -
Czerski L.,
Majors A.,
Ng T. C.,
Vijayakumar S.,
Weichselbaum R.
Publication year - 1993
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940060503
Subject(s) - matrigel , basement membrane , cell culture , magnetic resonance imaging , pathology , in vivo , chemistry , cell , cell growth , carcinoma , cancer research , materials science , nuclear medicine , nuclear magnetic resonance , angiogenesis , medicine , biology , radiology , physics , biochemistry , microbiology and biotechnology , genetics
Growth and magnetic resonance characteristics of a human squamous cell carcinoma SQ20B were studied in vivo as xenografts in nu/nu nude mice. Tumor cells injected subcutaneously in the flank using either Matrigel TM (MTG, an extract of basement membrane proteins) or growth medium (GM) as a vehicle were compared. Much higher tumor growth rates and cell density were observed with Matrigel TM than with GM implantation. Histology also showed that MTG implanted cells grew as vascularized solid tumors compared to GM tumors which formed cysts. As a result of increased cell density with the improved method, tumors as small as 0.3 cm 3 provide high S/N magnetic resonance spectra which yield smaller standard deviations with fewer experiments.