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Quantitative analysis of 1 H NMR detected proteins in the rat cerebral cortex in vivo and in vitro
Author(s) -
Kauppinen Risto A.,
Niskanen Timo,
Hakumäki Juhana,
Williams Stephen R.
Publication year - 1993
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940060403
Subject(s) - in vivo , macromolecule , chemistry , cerebrum , in vitro , mole , cerebral cortex , nuclear magnetic resonance , biochemistry , analytical chemistry (journal) , chromatography , biology , central nervous system , microbiology and biotechnology , endocrinology , physics , neuroscience
Spectral editing experiments were used to quantify CH 3 groups from macromolecular species in the chemical shift region from 1.2 to 1.4 ppm of rat cerebrum in vivo . Two peaks centred at 1.22 and 1.40 ppm were revealed when irradiation was positioned at 4.35 or 4.30 ppm. These peaks had lower saturation factors (1 vs. 1.72±0.10) than N ‐acetyl aspartate (NAA) and shorter T 2 [60±5.8 (1.22 ppm) and 51±2.2 (1.40 ppm) vs. 123±12 (NAA) ms]. The concentrations of the peaks at 1.22 and 1.40 ppm were calculated to be 0.65±0.09 and 1.37±0.18 mmol of CH 3 , equivalents/kg brain. Acid extract from cerebral corticies contained macromolecular peaks at the same chemical shifts with approximately the same area ratios to NAA as in vivo. These data show that the macromolecular peaks in the brain at TE >100 ms arise predominantly from proteins which are acid soluble. The assignment of macromolecular signals in the cerebral spectrum to a given polypeptide (thymosin β4 and histone H1) is discussed in the light of protein analyses of brain acid extracts.