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The effect of oestrogen ablation on the phospholipid metabolite content of primary and transplanted rat mammary tumours
Author(s) -
Smith T. A. D.,
Baluch S.,
Titley J. C.,
Ormerod M. G.,
Eccles S.,
Tombs A. J.,
Leach M. O.,
Griffiths J. R.,
McCready V. R.
Publication year - 1993
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940060307
Subject(s) - metabolite , phospholipid , ablation , chemistry , primary (astronomy) , mammary gland , endocrinology , medicine , biochemistry , cancer , membrane , breast cancer , physics , astronomy
The concentration of phospholipid metabolites was determined in chemical extracts from two types of rat mammary tumours and compared with proliferation data (S‐phase fraction). One of the tumours was an oestrogen‐sensitive transplanted tumour. In this tumour the concentration of phosphocholine (PC) and glycerophosphorylcholine (GPC) correlated strongly with the S‐phase fraction but not with the number of cells actively synthesizing DNA. Oestrogen ablation resulted in tumour regression. Regressing tumours contained less PC and more GPC than those actively growing. The other tumour was induced in rats by intravenous administration of N ‐methyl N ‐nitrosourea. Phosphoethanolamine (PE), PC and GPC levels were not associated with the S‐phase fraction in this tumour. Oestrogen ablation resulted in tumour regression. There was no significant difference between the regressing and growing tumours in PE, PC or GPC content.