Premium
A new high sensitivity 19 F probe for labeling cysteine groups of proteins
Author(s) -
Kalbitzer Hans Robert,
Rohr Gerhard,
Nowak Ewa,
Goody Roger S.,
Kuhn Winfried,
Zimmermann Erbert
Publication year - 1992
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940050605
Subject(s) - cysteine , chemistry , sensitivity (control systems) , radiochemistry , biochemistry , enzyme , electronic engineering , engineering
A novel iodoacetamide label 4‐perfluoro‐tert‐butyl‐phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19 F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; <100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40μM solution) can be detected easily in a single scan at 470 MHz.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom