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A new high sensitivity 19 F probe for labeling cysteine groups of proteins
Author(s) -
Kalbitzer Hans Robert,
Rohr Gerhard,
Nowak Ewa,
Goody Roger S.,
Kuhn Winfried,
Zimmermann Erbert
Publication year - 1992
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940050605
Subject(s) - cysteine , chemistry , sensitivity (control systems) , radiochemistry , biochemistry , enzyme , electronic engineering , engineering
A novel iodoacetamide label 4‐perfluoro‐tert‐butyl‐phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19 F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; <100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40μM solution) can be detected easily in a single scan at 470 MHz.