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Absolute quantification of 31 P liver metabolites in rat using an external reference and a surface spoiling magnetic field gradient
Author(s) -
De Bisschop Elke,
Luypaert Robert,
Annaert Guido,
Coremans Jean,
Osteaux Michel
Publication year - 1992
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940050604
Subject(s) - metabolite , calibration , magnetic field , field (mathematics) , chemistry , in vivo , nuclear magnetic resonance , approximation error , analytical chemistry (journal) , biological system , physics , chromatography , mathematics , mathematical analysis , biology , biochemistry , statistics , microbiology and biotechnology , quantum mechanics , pure mathematics
A simple method for measuring absolute concentrations of 31 P metabolites in rat liver using an external reference is described. It neutralizes systematic errors due to conductive losses by calibration of the matching capacitor and selects liver signals via an electrically driven, surface spoiling magnetic field gradient. The technique avoids the chemical shift problems associated with linear field gradient localization methods at 4.7 T and allows combinaton with the double standard method for absolute metabolite concentration determination. Application of the method to the in vivo measurement of the absolute concentrations of ATP and P i in eight rat livers yields results that are in good agreement with literature values. Absolute phosphomonoester concentrations were also obtained, but no literature data were available.