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Quantitation of metabolites in NMR spectra from isolated tissues, using 14 N spectroscopy and nitrate to determine tissue volume
Author(s) -
Wray S.,
Wilkie D. R.
Publication year - 1992
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940050306
Subject(s) - metabolite , chemistry , nuclear magnetic resonance spectroscopy , nmr spectra database , nitrate , volume (thermodynamics) , nuclear magnetic resonance , spectral line , analytical chemistry (journal) , chromatography , biochemistry , stereochemistry , physics , organic chemistry , quantum mechanics , astronomy
Quantification of metabolites is a goal of many biomedical NMR studies. To obtain absolute measurements of metabolite concentrations is often both difficult and time‐consuming. In this paper a method for determining metabolite concentrations directly is described and validated. It is applicable to studies of amphibian muscles, and with suitable precautions, to other isolated organs and tissues. The method is based upon using 14 N NMR and nitrate‐containing solutions to determine what fraction (F) of the sensitive volume of the RF coil is occupied by tissue. As the concentration of nitrate is known it can be used to calibrate other 14 N metabolites in the tissue. Moreover, once F is determined, it can be used to calibrate metabolites in spectra from other nuclei e.g., 31 P or 31 C. All that is required is that a spectrum from a standard for that nucleus is obtained. Thus this method does not require any “internal” (intrinsic to the tissue) standard, and is extremely quick and simple to use.