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Two‐dimensional 1 H spectroscopic imaging for evaluating the local metabolic response to focal ischemia in the conscious rat
Author(s) -
Peres Michel,
Bourgeois Dominique,
Roussel Simon,
Lefur Yann,
Devoulon Philippe,
Remy Chantal,
Barrere Bertrand,
Decorps Michel,
Pinard Elisabeth,
Riche Danièle,
Benabid AlimLouis,
Seylaz Jacques
Publication year - 1992
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940050104
Subject(s) - magnetic resonance imaging , nuclear magnetic resonance , magnetic resonance spectroscopic imaging , ischemia , creatine , choline , spin echo , voxel , stroke (engine) , chemistry , neuroimaging , medicine , nuclear medicine , radiology , physics , psychiatry , thermodynamics
Two‐dimensional 1 H spectroscopic imaging and magnetic resonance imaging were used to study focal ischemia induced by middle cerebral artery occlusion in rats. A water suppressing spin‐echo sequence was used at 4.7 T. Phase encoding during the spin‐echo delay (TE = 272 ms) yielded an 8 × 8 array of 35 μL voxels. The injured area of the brain had a higher lactate level and markedly lower N ‐acetyl aspartate, creatine and choline levels than did the non‐ischemic regions. The spectroscopic imaging data clearly showed the localization of the infarct, which agreed well with both magnetic resonance imaging and the histological data obtained post‐mortem. This study demonstrates the potential usefulness of combining magnetic resonance imaging and 1 H spectroscopic imaging for studying animal models of stroke, and indicates the suitability of the technique for further pharmacological approaches.