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23 Na, 59 Co and 2 H NMR studies of experimental acute pancreatitis
Author(s) -
Kushnir Tammar,
Kaplan Ofer,
Askenasy Nadir,
Navon Gil
Publication year - 1991
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940040405
Subject(s) - chemistry , sodium , reagent , volume (thermodynamics) , nuclear magnetic resonance , radiochemistry , organic chemistry , physics , quantum mechanics
Multinuclear MR was applied to the study of experimental acute pancreatitis. A continuous increase, up to 700%, of total, NMR visible, sodium content was detected during the development of the disease through the edematous stage to necrosis. The effect of the elevation of sodium content is amplified by an increased 23 Na NMR visibility during the disease. Interstitial and total water volume of pancreases were estimated using the distribution of Co(CN) 3 6 and D 2 O, by 50 Co and 2 H NMR, respectively. In healthy pancreases the interstitial compartment was found to comprise 35% of the tissue volume. In the diseased pancreases the penetration volume of Co(CN) 3 6 reached 90% of the total tissue volume, indicating extensive membrane injury. The time dependence of the 23 Na NMR in the presence of the shift reagent Na 7 Dy(tripolyphosphate) 2 .3NaCl provided additional evidence of the increased permeability of the membranes in the diseased organs.