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Cerebral metabolism of fluorodeoxyglucose measured with 19 F NMR spectroscopy
Author(s) -
Pouremad Reza,
Wyrwicz Alice M.
Publication year - 1991
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940040402
Subject(s) - mannose , chemistry , glucose 6 phosphate isomerase , glucose 6 phosphate , nuclear magnetic resonance spectroscopy , mannose 6 phosphate , dephosphorylation , phosphate , metabolism , fluorine 19 nmr , isomerase , isomerization , biochemistry , enzyme , stereochemistry , catalysis , phosphatase , receptor , growth factor
Brain extracts from rats given various doses of 2‐fluoro‐2‐deoxy‐D‐glucose ranging from 20 to 300 mg/kg were examined with 19 F NMR spectroscopy for fluorinated metabolic products and compared with the in vivo 19 F spectra. Based on the analysis of these spectra and comparison with synthetic reference compounds, 2‐fluoro‐2‐deoxy‐D‐glucose 6‐phosphate and 2‐fluoro‐2‐deoxy‐D‐mannose 6‐phosphate were identified as the major metabolites present. 2‐Fluoro‐2‐deoxy‐D‐6‐phosphogluconate was detected only at high doses of 2‐fluoro‐2‐deoxy‐D‐glucose administered, along with non‐phosphorylated 2‐fluoro‐2‐deoxy‐D‐glucose and 2‐fluoro‐2‐deoxy‐D‐mannose. Small amounts of two other compounds, which were not identified, were also observed. Presence of the 2‐fluoro‐2‐deoxy‐D‐mannose 6‐phosphate is the result of 2‐fluoro‐2‐deoxy‐D‐glucose 6‐phosphate isomerization at the C2 position catalyzed by phosphoglucose isomerase. Results from the in vitro enzymatic reaction provide support for this hypothesis. Presence of 2‐fluoro‐2‐deoxy‐D‐mannose may be the result of 2‐fluoro‐2‐deoxy‐D‐glucose isomerization at the C2 position catalyzed by glucose isomerase or the result of the dephosphorylation of the 2‐fluoro‐2‐deoxy‐D‐glucose 6‐phosphate.