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Quantification of glutamate, glutamine, and other metabolites in In Vivo proton NMR spectroscopy
Author(s) -
Bovée W. M. M. J.
Publication year - 1991
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940040209
Subject(s) - pulse sequence , nuclear magnetic resonance , in vivo , spins , glutamine , nuclear magnetic resonance spectroscopy , pulse (music) , chemistry , spectroscopy , proton , sequence (biology) , line (geometry) , spectral line , intensity (physics) , biological system , analytical chemistry (journal) , physics , biology , optics , mathematics , amino acid , chromatography , biochemistry , nuclear physics , genetics , quantum mechanics , astronomy , detector , condensed matter physics , geometry
A reliable quantification of in vivo 1 H MRS spectra is hampered by extensive line overlap, by intensity distortions due to the water suppressing pulse sequence and complex modulation patterns of J ‐coupled spins, and by deviations between the experimental and model lineshapes in computer fitting programs. By correcting the experimental lineshape, using a suitable pulse sequence, and incorporating prior knowledge about the spin systems and the intensity distortions, a sufficient quantification is possible. If reliable. T 2 values are available absolute concentrations can be determined.