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A quantitative analysis of the accuracy of In Vivo pH measurements with 31 P NMR spectroscopy: Assessment of pH measurement methodology
Author(s) -
Madden A.,
Leach M. O.,
Sharp J. C.,
Collins D. J.,
Easton D.
Publication year - 1991
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940040102
Subject(s) - nuclear magnetic resonance spectroscopy , spectroscopy , chemistry , in vivo , analytical chemistry (journal) , intracellular ph , accuracy and precision , gaussian , observational error , biological system , nuclear magnetic resonance , chromatography , mathematics , statistics , computational chemistry , intracellular , physics , stereochemistry , biochemistry , microbiology and biotechnology , quantum mechanics , biology
31 P NMR spectroscopy provides a means of monitoring intracellular pH (pH i ). In order to determine the significance of observed pH changes, an assessment of the measurement uncertainty is required. This paper considers the possible sources of error involved, firstly in chemical shift assignment and, secondly, in relating chemical shift to pH. A statistical analysis of these errors is presented and an expression for the accuracy of pH measurements obtained using in vivo 31 P NMR spectroscopy is derived. Computer simulations and clinical measurements are considered to determine the likely error in clinical measurements. A comparison of pH measurement methodology using a centre‐of‐gravity calculation, Gaussian and Lorentzian fitting is also presented and discussed.