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Metabolic heterogeneity in RIF‐1 tumours detected in vivo by 31 P NMR spectroscopy
Author(s) -
Bhujwalla Z. M.,
Blackband S. J.,
Wehrle J. P.,
Grossman S.,
Eller S.,
Glickson J. D.
Publication year - 1990
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940030507
Subject(s) - necrosis , in vivo , chemistry , phosphate , nuclear magnetic resonance spectroscopy , distribution (mathematics) , phosphorus , quantitative analysis (chemistry) , nuclear magnetic resonance , pathology , biology , biochemistry , chromatography , medicine , stereochemistry , mathematical analysis , microbiology and biotechnology , mathematics , physics , organic chemistry
The spatial distribution of phosphate metabolites and pH within subcutaneously implanted RIF‐1 tumours was determined by 1‐dimensional phosphorus chemical shift imaging. 31 P spectra from two to three 4 mm thick cross‐sectional slices were obtained for each tumour. Quantitative morphometry was used to estimate the amount of necrosis within those slices. A spatially heterogeneous distribution of phosphate metabolites and pH was detected in most of the tumours. Levels of necrosis ranged from 0–24% for the tumours in this study. There was no significant correlation between the extent of necrosis over this range and levels of metabolites or pH, suggesting that factors besides necrosis can contribute to spectral heterogeneity.