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Multiple quantum filtered 23 Na NMR spectroscopy in the perfused heart
Author(s) -
Payne Geoffrey S.,
Seymour AnneMarie L.,
Styles Peter,
Radda George K.
Publication year - 1990
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940030307
Subject(s) - extracellular , reagent , intracellular , nuclear magnetic resonance spectroscopy , chemistry , relaxation (psychology) , nuclear magnetic resonance , quenching (fluorescence) , spectroscopy , biophysics , analytical chemistry (journal) , biochemistry , chromatography , biology , physics , fluorescence , stereochemistry , neuroscience , quantum mechanics
It has been proposed that multiple quantum filtered (MQF) 23 Na NMR spectroscopy may distinguish between sodium in the intra‐ and extracellular spaces, in vivo , and without the requirement for toxic shift reagents. We have investigated the origin of such signals in perfused rat hearts, using shift reagents to discriminate between NMR signals from the two compartments. We find that the observed MQF signal arises predominantly from the extracellular space in the normal heart, and from the intracellular space in the ischaemic heart. However, the quenching effect of the shift reagent may hide some of the extracellular MQF signal, especially during ischaemia. Estimates of intracellular and extracellular relaxation times are obtained and possible mechanisms of the interaction discussed.