31 P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium | Zendy

Narayan K. Shankar | Zendy; Moress E. A. | Zendy; Chatham J. C. | Zendy; Barker P. B. | Zendy

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31 P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium

Author(s) -

Narayan K. Shankar,

Moress E. A.,

Chatham J. C.,

Barker P. B.

Publication year - 1990

Publication title -

nmr in biomedicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.278

H-Index - 114

eISSN - 1099-1492

pISSN - 0952-3480

DOI - 10.1002/nbm.1940030104

Subject(s) - intracellular , nuclear magnetic resonance spectroscopy , phosphate , chemistry , cell , perfusion , inorganic phosphate , metabolism , calcium , calcium alginate , biophysics , cell culture , biochemistry , nuclear magnetic resonance , biology , stereochemistry , organic chemistry , medicine , genetics , cardiology , physics

A simple technique permitting long term 31 P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/P i ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.

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