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31 P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium
Author(s) -
Narayan K. Shankar,
Moress E. A.,
Chatham J. C.,
Barker P. B.
Publication year - 1990
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940030104
Subject(s) - intracellular , nuclear magnetic resonance spectroscopy , phosphate , chemistry , cell , perfusion , inorganic phosphate , metabolism , calcium , calcium alginate , biophysics , cell culture , biochemistry , nuclear magnetic resonance , biology , stereochemistry , organic chemistry , medicine , genetics , cardiology , physics
A simple technique permitting long term 31 P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/P i ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.