Premium
Multilabeled 13 C substrates as probes in In vivo 13 C and 1 H NMR spectroscopy
Author(s) -
Künnecke Basil,
Cerdan Sebastian
Publication year - 1989
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940020516
Subject(s) - homonuclear molecule , nuclear magnetic resonance spectroscopy , spectroscopy , chemistry , nuclear magnetic resonance , in vivo , proton , analytical chemistry (journal) , stereochemistry , molecule , physics , chromatography , biology , organic chemistry , quantum mechanics , microbiology and biotechnology
The potential use of 13 C multilabeled substrates has been studied in biological applications using in vivo and in vitro proton and 13 C NMR spectroscopy. In 13 C NMR spectroscopy, multilabeled compounds allow the simultaneous observation of several nuclei or increase distinctly the signal to noise ratio due to a higher degree of enrichment. Contiguous labeling of substrates leads to homonuclear 13 C‐ 13 C spin couplings and provides a simple means to distinguish between endogenous stores of metabolites and metabolites derived from added substrates.