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Localized 13 C‐{ 1 H} NMR of rat liver In vivo using surface‐spoiling gradients
Author(s) -
Chen Wei,
Ackerman Joseph J. H.
Publication year - 1989
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940020515
Subject(s) - in vivo , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , laser linewidth , chemistry , spectroscopy , resolution (logic) , glycogen , analytical chemistry (journal) , physics , materials science , optics , biology , biochemistry , laser , microbiology and biotechnology , quantum mechanics , chromatography , artificial intelligence , computer science
An NMR spectroscopy localization method that employs an inhomogeneous surface‐spoiling magnetic field gradient has been applied to detect the 13 C‐{ 1 H} signals from deep‐lying liver tissue of rat in vivo . The method requires small gradient driving currents (less than 1 amp) and short gradient periods (˜0.5–1.6 ms). Thus, it is not likely to suffer from induced eddy currents and can detect short T 2 resonances. We demonstrate that by employing a pulsed surface‐spoiling gradient one can eliminate “contaminating” signals from the surface‐lying tissues (muscle, fat and skin) while still maintaining the resonance linewidth resolution for the deep‐lying tissue of interest (liver). The feasibility of using this localization approach to trace glucose conversion into glycogen is shown.