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31 P NMR spectroscopic study of bioenergetic changes in radiation‐induced fibrosarcoma‐1 after radiation therapy
Author(s) -
Rajan S. Sunder,
Wehrle Janna P.,
Li ShiJiang,
Steen R. Grant,
Glickson Jerry D.
Publication year - 1989
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940020406
Subject(s) - fibrosarcoma , phosphomonoesters , phosphocreatine , in vivo , chemistry , radiation therapy , clonogenic assay , irradiation , toxicity , bioenergetics , cancer research , nuclear medicine , medicine , biology , pathology , in vitro , biochemistry , energy metabolism , physics , microbiology and biotechnology , nuclear physics , mitochondrion
The effects of localized γ‐irradiation on the in vivo 31 P NMR spectra of RIF‐1 tumors grown subcutaneously in C3H/HeN mice have been examined before and during the week after treatment. Increases in the ratio of phosphocreatine (PCr) to inorganic phosphate (P i ) and in tumor pH, and decreases in the ratio of P i to the b̃ phosphorus resonance of the nucleotide triphosphates (b̃NTP) were observed in irradiated tumors. The time course of changes in the 31 P spectrum following treatment was the opposite of the pattern during untreated growth, and the magnitude and duration of the changes increased with increasing radiation dose, decreasing clonogenic cell survival and increasing growth delay. To examine the possibility that nontherapeutic systemic effects of the tumor irradiation were responsible for the changes observed, a number of animals bearing two tumors were examined. One tumor on each mouse was selectively irradiated. Changes in tumor volume, P i /b̃NTP, PCr/P i , the ratio of phosphomonoesters to b̃NTP, and tumor pH were all significantly different in the treated compared to the untreated tumor on each animal, indicating that these changes in 31 P NMR spectra were a response to radiation therapy and not a systemic response to radiation toxicity.