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Characterization of the transport of the nonelectrolyte dimethyl methylphosphonate across the red cell membrane
Author(s) -
Potts Jennifer R.,
Kirk Kiaran,
Kuchel Philip W.
Publication year - 1989
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940010408
Subject(s) - dimethyl methylphosphonate , chemistry , phloretin , thiourea , membrane , ionophore , urea , permeability (electromagnetism) , nuclear chemistry , inorganic chemistry , organic chemistry , biochemistry
We have refined the method for measuring the equilibrium exchange of the neutral phosphoryl compound dimethyl methylphosphonate across the human red cell membrane using 31 P NMR spectroscopy. Using this improved technique we measured a permeability coefficient of dimethyl methylphosphonate of 9.47 ± 1.01 × 10 –5 cm s –1 at a concentration of 164 m M , at 25 °C. The transport at 25 °C was not saturable up to a concentration of 600 m M and was not inhibited by 4,4′‐diisothiocyano‐2,2′‐disulfonic acid, p ‐chloromercuriphenyl sulfonic acid, copper ions or phloretin, or by thiourea or urea at 16 °C. The permeability was enhanced by butanol and phloretin.