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In vivo 31 P NMR spectroscopy of the rat cerebral cortex during acute hepatic encephalopathy
Author(s) -
Deutz N. E. P.,
Chamuleau R. A. F. M.,
De Graaf A. A.,
Bovée W. M. M. J.,
De Beer R.
Publication year - 1988
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940010208
Subject(s) - phosphocreatine , glutamine , hepatic encephalopathy , amino acid , medicine , encephalopathy , cerebral cortex , endocrinology , chemistry , ischemia , intracellular , intracellular ph , pathophysiology , pathogenesis , glutamate receptor , cortex (anatomy) , biology , biochemistry , energy metabolism , neuroscience , cirrhosis , receptor
During the development of acute hepatic encephalopathy, induced by acute liver ischemia, changes in brain 31 P NMR spectra and EEG spectra were studied over 8:45 h in eight rats. At the end of this period the brain amino acid concentrations were determined. The results were compared with the same measurements in four normal and three portacaval shunted rats. Signs of acute HE, as judged by the EEG left index, started 5 h after the induction of acute liver ischemia. No accompanying significant changes in the cortical relative phosphocreatine and ATP concentration and intracellular pH were observed. The cortical relative P i concentration had only slightly increased at t = 8 h. The concentrations of almost all measured brain amino acids, especially glutamine had increased at t = 8:45 h. At t = 8 h, rats with very severe HE had a small, but significant decrease of brain ATP concentrations. Their brain amino acid concentrations were more disturbed than in rats with less severe HE. It is concluded that a change in the cortical cerebral energy rich phosphate concentration is not an important pathophysiological mechanism during the development of acute HE. The observed changes in brain amino acids concentrations could be either part of a multifactorial pathogenesis or could be epiphenomena.