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Factors affecting 1 h NMR spectra of blood plasma: cancer, diet and freezing
Author(s) -
Bell J. D.,
Brown J. C. C.,
Norman R. E.,
Sadler P. J.,
Newell D. R.
Publication year - 1988
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940010206
Subject(s) - spectral line , chemistry , nuclear magnetic resonance , plasma , nmr spectra database , pulse sequence , molecule , lipoprotein , cholesterol , physics , biochemistry , organic chemistry , quantum mechanics , astronomy
Single pulse and Hahn spin‐echo 400 and 500 MHz 1 H NMR spectra from normal subjects and cancer patients are reported. No significant difference was found between the average —CH 3 and —(CH 2 ) n — lipoprotein linewidths of the two groups in contrast to a previous report. Such measurements are shown to be complicated by freezing and thawing of samples which broadens lipoprotein resonances, and by eating which sharpens them. Deconvolution of the composite lipoprotein signals enables these effects to be interpreted. Other notable features of plasma spectra are discussed including resonances for ‘acute‐phase’ glycoproteins, ketone bodies and other small molecules.