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Phosphate metabolites in rat skin
Author(s) -
Stubbs Marion,
Vanstapel Florent,
Rodrigues Loreta M.,
Griffiths John R.
Publication year - 1988
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1940010109
Subject(s) - phosphomonoesters , phosphocreatine , creatine , phosphate , chemistry , nmr spectra database , phospholipid , anatomy , microbiology and biotechnology , biochemistry , biology , endocrinology , inorganic phosphate , spectral line , physics , astronomy , membrane , energy metabolism
A model for studying the 31 P NMR spectrum of rat skin without contribution from other tissue signals has been developed by creating a skin pedicle. 31 P NMR spectra were obtained with a solenoidal coil, which was separated from the flank of the rat by a Faraday shield. Phosphomonoesters, inorganic phosphate (P i ) (1.63 ± 0.12 m̈mol per g wet wt), phosphodiesters, phosphocreatine (PCr) (1.4 ± 0.12 m̈mol per g wet wt) and ATP (1.35 ± 0.22 m̈mol per g wet wt) were observed, superimposed on broader signals, probably due to phospholipids. Extracts of freeze‐clamped pedicles contained concentrations of phosphorus metabolites similar to those seen by NMR. The exception was P i which was twofold higher in the extract. The presence of the broader phospholipid contribution suggests that the signals did not arise solely from the panniculus carnosus muscle of rat skin, although this muscle was evident on histological examination of the pedicles. In extracts of normal rat skin levels of creatine, ATP, ADP and P i were similar to those of pedicles, whereas PCr was about twofold higher. Signals from rat skin are likely to contribute to spectra of subcutaneous organs and tumours. Two kinds of rat hepatoma that contained no PCr frequently gave PCr signals from the overlying skin, whereas in three other subcutaneous tumours the contribution from skin was negligible.