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In vivo MRI of olfactory ensheathing cell grafts and regenerating axons in transplant mediated repair of the adult rat optic nerve
Author(s) -
Sandvig Ioanna,
Thuen Marte,
Hoang Linh,
Olsen Øystein,
Sardella Thomas CP,
Brekken Christian,
Tvedt Kåre E,
Barnett Susan C,
Haraldseth Olav,
Berry Martin,
Sandvig Axel
Publication year - 2012
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1778
Subject(s) - remyelination , olfactory ensheathing glia , optic nerve , regeneration (biology) , in vivo , retinal ganglion cell , magnetic resonance imaging , transplantation , pathology , anatomy , chemistry , biology , medicine , microbiology and biotechnology , neuroscience , central nervous system , myelin , olfactory bulb , surgery , radiology
The purpose of the present study was to use magnetic resonance imaging (MRI) as a tool for monitoring transplant‐mediated repair of the adult rat visual pathway. We labelled rat olfactory ensheathing cells (OECs) using micron‐sized particles of iron oxide (MPIO) and transplanted them by: i) intravitreal injection ( ivit ) and ii) intra‐optic nerve (ON) injection ( iON ) in adult rats with ON crush (ONC) injury. We applied T 2 ‐weighted MRI and manganese‐enhanced MRI (MEMRI) to visualise transplanted cells and ON axons at specific times after injury and cell engraftment. Our findings demonstrate that ivit MPIO‐labelled OECs are unequivocally detected by T 2 ‐weighted MRI in vivo and that the T 1 ‐weighted 3D FLASH sequence applied for MEMRI facilitates simultaneous visualisation of Mn 2+− enhanced regenerating retinal ganglion cell (RGC) axons and MPIO‐labelled OEC grafts. Furthermore, analysis of MRI data and ultrastructural findings supports the hypothesis that iON OEC transplants mediate regeneration and remyelination of RGC axons post injury. Copyright © 2011 John Wiley & Sons, Ltd.