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In vivo detection of intermediate metabolic products of [1‐ 13 C]ethanol in the brain using 13 C MRS
Author(s) -
Xiang Yun,
Shen Jun
Publication year - 2011
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1653
Subject(s) - in vivo , ethanol , chemistry , glutamine , acetaldehyde , alcohol , glutamate receptor , ethanol metabolism , glutamatergic , metabolism , biochemistry , biology , amino acid , receptor , microbiology and biotechnology
Abstract In this study, in vivo 13 C MRS was used to investigate the labeling of brain metabolites after intravenous administration of [1‐ 13 C]ethanol. After [1‐ 13 C]ethanol had been administered systemically to rats, 13 C labels were detected in glutamate, glutamine and aspartate in the carboxylic and amide carbon spectral region. 13 C‐labeled bicarbonate HCO 3 –(161.0 ppm) was also detected. Saturating acetaldehyde C1 at 207.0 ppm was found to have no effect on the ethanol C1 (57.7 ppm) signal intensity after extensive signal averaging, providing direct in vivo evidence that direct metabolism of alcohol by brain tissue is minimal. To compare the labeling of brain metabolites by ethanol with labeling by glucose, in vivo time course data were acquired during intravenous co‐infusion of [1‐ 13 C]ethanol and [ 13 C 6 ]‐ D ‐glucose. In contrast with labeling by [ 13 C 6 ]‐ D ‐glucose, which produced doublets of carboxylic/amide carbons with a J coupling constant of 51 Hz, the simultaneously detected glutamate and glutamine singlets were labeled by [1‐ 13 C]ethanol. As 13 C labels originating from ethanol enter the brain after being converted into [1‐ 13 C]acetate in the liver, and the direct metabolism of ethanol by brain tissue is negligible, it is suggested that orally or intragastrically administered 13 C‐labeled ethanol may be used to study brain metabolism and glutamatergic neurotransmission in investigations involving alcohol administration. In vivo 13 C MRS of rat brain following intragastric administration of 13 C‐labeled ethanol is demonstrated. Published in 2011 by John Wiley & Sons, Ltd.