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High‐field MRI of single histological slices using an inductively coupled, self‐resonant microcoil: application to ex vivo samples of patients with Alzheimer's disease
Author(s) -
Nabuurs Rob J. A.,
Hegeman Ingrid,
Natté Remco,
van Duinen Sjoerd G.,
van Buchem Mark A.,
van der Weerd Louise,
Webb Andrew G.
Publication year - 2011
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1598
Subject(s) - ex vivo , microcoil , biomedical engineering , pathology , in vivo , staining , histology , magnetic resonance imaging , materials science , medicine , radiology , biology , physics , microbiology and biotechnology , quantum mechanics , electromagnetic coil
A simple inductively coupled microcoil has been designed to image tissue samples placed on a microscope slide, samples which can subsequently be stained histologically. As the exact same tissue is used for MRI and histology, the two data sets can be compared without the need for complicated image registration techniques. The design can be integrated into any MRI system using existing commercial hardware. Compared with a commercial 25‐mm‐diameter birdcage, the signal‐to‐noise ratio was increased by a factor of 3.8, corresponding to an approximate 15‐fold reduction in the data acquisition time. An example is shown of ex vivo samples from patients with Alzheimer's disease, in which the coregistration of highly sensitive iron staining and amyloid‐β deposits is confirmed. Copyright © 2010 John Wiley & Sons, Ltd.