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Compatibility of superparamagnetic iron oxide nanoparticle labeling for 1 H MRI cell tracking with 31 P MRS for bioenergetic measurements
Author(s) -
Zhang Zhuoli,
Hancock Brynne,
Leen Stephanie,
Ramaswamy Sharan,
Sollott Steven J.,
Boheler Kenneth R.,
Juhaszova Magdalena,
Lakatta Edward G.,
Spencer Richard G.,
Fishbein Kenneth W.
Publication year - 2010
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1545
Subject(s) - bioenergetics , chemistry , intracellular , superparamagnetism , nuclear magnetic resonance , in vivo , iron oxide , cell , nanoparticle , pyrophosphate , viability assay , biophysics , materials science , biochemistry , nanotechnology , magnetization , enzyme , biology , physics , microbiology and biotechnology , organic chemistry , quantum mechanics , magnetic field , mitochondrion
Labeling of cells with superparamagnetic iron oxide nanoparticles permits cell tracking by 1 H MRI while 31 P MRS allows non‐invasive evaluation of cellular bioenergetics. We evaluated the compatibility of these two techniques by obtaining 31 P NMR spectra of iron‐labeled and unlabeled immobilized C2C12 myoblast cells in vitro. Broadened but usable 31 P spectra were obtained and peak area ratios of resonances corresponding to intracellular metabolites showed no significant differences between labeled and unlabeled cell populations. We conclude that 31 P NMR spectra can be obtained from cells labeled with sufficient iron to permit visualization by 1 H imaging protocols and that these spectra have sufficient quality to be used to assess metabolic status. This result introduces the possibility of using localized 31 P MRS to evaluate the viability of iron‐labeled therapeutic cells as well as surrounding host tissue in vivo. Published in 2010 by John Wiley & Sons, Ltd.

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