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Comparative 1 H NMR studies of saturation transfer in copolymer gels and mouse lenses
Author(s) -
Nakamura Koji,
Sogami Masaru,
Era Seiichi,
Matsushima Shigeru,
Kinosada Yasutomi
Publication year - 2010
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1499
Subject(s) - copolymer , saturation (graph theory) , chemistry , polymer chemistry , polymer , organic chemistry , mathematics , combinatorics
Abstract Saturation transfer in cross‐linked copolymer gels and excised intact and perforating trauma‐induced cataract mouse lenses (4‐ or 8‐week‐old) were studied using intermolecular cross‐relaxation rates (1/ T IS (H 2 O); 1/ T IS ), monitored with f 2 ‐irradiation at −8.79, −4.00, and 7.13 ppm (γH 2 /2 π ∼ 69 Hz). [1] The 1/ T IS (7.13 ppm) vs dry weight [W (%)] profiles for hydrophilic copolymer gels were far steeper than those for hydrophobic copolymer gels, indicating the participation of an amount of bound water and a number of copolymer hydroxyl groups in the saturation transfer process. In contrast, the 1/ T IS (−8.79 ppm) vs W (%) profiles exhibited little difference between the hydrophilic and hydrophobic copolymer gels, indicating the major participation of molecular rigidity, i.e. W (%) in the saturation transfer process. [2] The 1/ T IS (7.13 ppm) values for cataractous mouse lenses were larger than those for intact lenses, indicating the formation of large, immobile lens protein associates or aggregates containing a sufficient amount of bound water for the saturation transfer. [3] The 1/ T IS (7.13 ppm) vs W (%) profiles for the hydrophilic copolymer gels exhibited similar characteristics to the intact and cataractous mouse lenses with regard to the saturation transfer process. Copyright © 2010 John Wiley & Sons, Ltd.