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Monitoring angiogenesis in soft‐tissue engineered constructs for calvarium bone regeneration: an in vivo longitudinal DCE‐MRI study
Author(s) -
Beaumont Marine,
DuVal Marc G.,
Loai Yasir,
Farhat Walid A.,
Sándor George K.,
Cheng HaiLing Margaret
Publication year - 2010
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1425
Subject(s) - in vivo , regeneration (biology) , angiogenesis , soft tissue , biomedical engineering , medicine , pathology , biology , microbiology and biotechnology , cancer research
Tissue engineering is a promising technique for bone repair and can overcome the major drawbacks of conventional autogenous bone grafting. In this in vivo longitudinal study, we proposed a new tissue‐engineering paradigm: inserting a biological soft‐tissue construct within the bone defect to enhance angiogenesis for improved bone regeneration. The construct acts as a resorbable scaffold to support desired angiogenesis and cellular activity and as a vector of vascular endothelial growth factor, known to promote both vessel and bone growth. Dynamic contrast‐ enhanced magnetic resonance imaging was performed to investigate and characterize angiogenesis necessary for bone formation following the proposed paradigm of inserting a VEGF‐impregnated tissue‐engineered construct within the critical‐sized calvarial defect in the membranous parietal bone of the rabbit. Results show that a model‐free quantitative approach, the normalized initial area under the curve metric, provides sensitive and reproducible measures of vascularity that is consistent with known temporal evolution of angiogenesis during bone regeneration. Copyright © 2009 John Wiley & Sons, Ltd.