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Manganese‐enhanced MRI visualizes V1 in the non‐human primate visual cortex
Author(s) -
Bock Nicholas A.,
Kocharyan Ara,
Silva Afonso C.
Publication year - 2009
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1384
Subject(s) - marmoset , visual cortex , callithrix , cortex (anatomy) , in vivo , temporal cortex , primate , neuroscience , anatomy , biology , chemistry , paleontology , microbiology and biotechnology
MRI at 7 Tesla has been used to investigate the accumulation of manganese in the occipital cortex of common marmoset monkeys ( Callithrix jacchus ) after administering four fractionated injections of 30 mg/kg MnCl 2 · 4H 2 O in the tail vein. We found a statistically significant decrease in T 1 in the primary (V1) and secondary (V2) areas of the visual cortex caused by an accumulation of manganese. The larger T 1 shortening in V1 (Δ T 1 = 640 ms) relative to V2 (Δ T 1 = 490 ms) allowed us to robustly detect the V1/V2 border in vivo using heavily T 1 ‐weighted MRI. Furthermore, the dorso‐medial (DM) and middle‐temporal (MT) areas of the visual pathway could be identified by their T 1 ‐weighted enhancement. We showed by comparison to histological sections stained for cytochrome oxidase (CO) activity that the extent of V1 is accurately identified throughout the visual cortex by manganese‐enhanced MRI (MEMRI). This provides a means of visualizing functional cortical regions in vivo and could be used in longitudinal studies of phenomena such as cortical plasticity, and for non‐destructive localization of cortical regions to guide in the implementation of functional techniques. Published in 2009 by John Wiley & Sons, Ltd.