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Macrophage physiological function after superparamagnetic iron oxide labeling
Author(s) -
Hsiao JongKai,
Chu HungHao,
Wang YuHsiu,
Lai ChihWei,
Chou PiTai,
Hsieh SungTsang,
Wang JawLin,
Liu HonMan
Publication year - 2008
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1260
Subject(s) - nitric oxide , macrophage , phagocytosis , chemistry , organelle , microbiology and biotechnology , function (biology) , secretion , cytoplasm , biophysics , biochemistry , biology , in vitro , organic chemistry
Our goal was to analyze the changes in morphology and physiological function (phagocytosis, migratory capabilities, humoral and cellular response, and nitric oxide secretion) of murine macrophages after labeling with a clinically used superparamagnetic iron oxide (SPIO), ferucarbotran. In SPIO‐treated macrophages, nanoparticles were taken up in the cytoplasm and accumulated in a membrane‐bound organelle. Macrophage proliferation and viability were not modified after SPIO labeling. Phagocytic function decreased after labeling with only 10 µg Fe/mL SPIO, whereas other functions including migration and production of tumor necrosis factor‐ α and nitric oxide increased at the highest SPIO concentration (100 µg Fe/mL). Copyright © 2008 John Wiley & Sons, Ltd.