Premium
Blood–brain barrier permeability to manganese and to Gd‐DOTA in a rat model of transient cerebral ischaemia
Author(s) -
Grillon Emmanuelle,
Provent Peggy,
Montigon Olivier,
Segebarth Christoph,
Rémy Chantal,
Barbier Emmanuel L.
Publication year - 2008
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1206
Subject(s) - extravasation , occlusion , effective diffusion coefficient , blood–brain barrier , medicine , ischemia , evans blue , middle cerebral artery , magnetic resonance imaging , anesthesia , nuclear medicine , chemistry , pathology , central nervous system , radiology
Loss of integrity of the blood–brain barrier (BBB) and brain swelling is a potentially lethal complication of reperfusion in human stroke. To assess the time course of BBB modifications, we performed angiography, diffusion‐weighted imaging, T 1‐weighted ( T 1 W) imaging and T 1 mapping, and monitored acute changes after middle cerebral artery occlusion and recanalization in rats (n = 27). The animals were grouped according to the duration of occlusion: 30 min (group A, n = 8), 1 h 30 min (group B, n = 9), and 2 h 30 min (group C, n = 10). For 17 animals (four in group A, six in group B, and seven in group C), MnCl 2 and dimeglumine gadoterate (Gd‐DOTA) were injected at 13 min and 34 min after recanalization, respectively. The 10 remaining animals (control groups) underwent the same acquisition protocols, but no contrast agents were injected. Cell damage was determined 1 h after recanalization on haematoxylin and eosin‐stained sections. Our results indicate that in the middle cerebral artery occlusion model in the rat, changes in BBB permeability assessed by contrast agent extravasation occur within the first hour of reperfusion, even after an occlusion period not exceeding 30 min. No differences between BBB permeability to Gd‐DOTA and Mn 2+ were detected in our experimental conditions. The reduction in apparent diffusion coefficient during occlusion appears to be a good predictor of BBB modifications after reperfusion in this model. Copyright © 2007 John Wiley & Sons, Ltd.