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Gd(III)‐EPTPAC 16 , a new self‐assembling potential liver MRI contrast agent: in vitro characterization and in vivo animal imaging studies
Author(s) -
Torres Suzana,
Prata Maria I. M.,
Santos Ana C.,
André João P.,
Martins José A.,
Helm Lothar,
Tóth Éva,
GarcíaMartín Maria L.,
Rodrigues Tiago B.,
LópezLarrubia Pilar,
Cerdán Sebastián,
Geraldes Carlos F. G. C.
Publication year - 2008
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1194
Subject(s) - sonication , in vivo , biodistribution , chemistry , dispersity , chelation , mri contrast agent , aqueous solution , in vitro , nuclear chemistry , biophysics , gadolinium , chromatography , biochemistry , inorganic chemistry , organic chemistry , microbiology and biotechnology , biology
The recently reported amphiphilic chelate, [Gd(EPTPAC 16 )(H 2 O)] 2− , forms supramolecular aggregates in aqueous solution by self‐assembly of the monomers with a relaxometrically determined critical micellar concentration (CMC) of 0.34 mM. The effect of sonication on the aggregate size was characterized by dynamic light scattering and relaxometry, indicating the presence of premicellar aggregates and an overall decrease in aggregate size and polydispersity upon sonication, slightly below the CMC. {[ 153 Sm](EPTPAC 16 )(H 2 O)} 2− radiotracer was evaluated in vivo from γ scintigraphy and biodistribution in Wistar rats. It was found to depend strongly on the sample concentration, below or above the CMC, and its sonication, in a way that correlates with the effect of the same factors on the size of the aggregates formed in solution. Below CMC, the very large aggregates of the [ 153 Sm] 3+ ‐labeled chelate were persistently and mainly taken up by the lungs, and also by the macrophage‐rich liver and spleen. Sonication of this solution led to loss of the lung uptake. Above CMC, the metal chelate was mainly taken up by the liver, with very little uptake by the spleen and lungs. In vivo , dynamic contrast‐enhanced (DCE)‐MRI evaluation of the micellar [Gd(EPTPAC 16 )(H 2 O)] 2− compound in Wistar rats showed a persistent hepatic positive‐contrast effect in T 1 ‐weighted images, qualitatively similar to the clinically established Gd III ‐based hepatobiliary‐selective agents. No enhancement effect was observed in the lungs because of the scarcity of mobile protons in this organ, despite the scintigraphic evidence of significant lung retention of the [ 153 Sm] 3+ ‐labeled chelate at concentrations below the CMC. Copyright © 2007 John Wiley & Sons, Ltd.