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Cell labeling for magnetic resonance imaging with the T 1 agent manganese chloride
Author(s) -
Aoki Ichio,
Takahashi Yoshiyuki,
Chuang KaiHsiang,
Silva Afonso C.,
Igarashi Takehito,
Tanaka Chuzo,
Childs Richard W.,
Koretsky Alan P.
Publication year - 2006
Publication title -
nmr in biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 114
eISSN - 1099-1492
pISSN - 0952-3480
DOI - 10.1002/nbm.1000
Subject(s) - propidium iodide , flow cytometry , cytotoxic t cell , chemistry , annexin , apoptosis , microbiology and biotechnology , incubation , cell , in vitro , biophysics , cancer research , biochemistry , biology , programmed cell death
There is growing interest in using MRI to track cellular migration. To date, most work in this area has been performed using ultra‐small particles of iron oxide. Immune cells are difficult to label with iron oxide particles. The ability of adoptively infused tumor specific T cells and N cells to traffic to the tumor microenvironment may be a critical determinant of their therapeutic efficacy. We tested the hypothesis that lymphocytes and B cells would label with MnCl 2 to a level that would allow their detection by T 1 ‐weighted MRI. Significant signal enhancement was observed in human lymphocytes after a 1 h incubation with 0.05–1.0 mM MnCl 2 . A flow cytometry‐based evaluation using propidium iodide and Annexin V staining showed that lymphocytes did not undergo apoptosis or necrosis immediately after and 24 h following a 1 h incubation with up to 1.0 mM MnCl 2 . Importantly, NK cells and cytotoxic T cells maintained their in vitro killing capacity after being incubated with up to 0.5 mM MnCl 2 . This is the first report to describe the use of MnCl 2 to label lymphocytes. Our data suggests MnCl 2 might be an alternative to iron oxide cell labeling for MRI‐based cell migration studies. Copyright © 2006 John Wiley & Sons, Ltd.