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Hydrodistention does not alter bladder gene expression profiles in patients with non‐Hunner lesion interstitial cystitis/bladder pain syndrome
Author(s) -
Xu Raymond,
Schachar Jeffrey,
Evans Robert J.,
Matthews Catherine A.,
Badlani Gopal,
Walker Stephen J.
Publication year - 2021
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.24680
Subject(s) - interstitial cystitis , medicine , bladder pain syndrome , gene expression , biopsy , bladder outlet obstruction , gene expression profiling , urology , microarray , urinary bladder neck obstruction , gene , pathology , urinary system , biology , prostate , biochemistry , cancer
Aims Gene expression profiling of bladder biopsies in patients with interstitial cystitis/bladder pain syndrome (IC/BPS), typically obtained following therapeutic bladder hydrodistention (HOD), is used to improve our understanding of molecular phenotypes. The objective of this study was to determine if the HOD procedure itself impacts the biopsy gene expression profile and, by extension, whether biopsies from non‐HOD bladders are appropriate controls. Methods Bladder biopsies were obtained just before HOD and immediately following HOD from 10 consecutively recruited IC/BPS patients undergoing therapeutic HOD. Biopsies were also obtained from four non‐IC/BPS patients who did not undergo HOD (controls). Total RNA was isolated from each of the 24 samples and used to query whole‐genome microarrays. Differential gene expression analysis was performed to compare expression profiles of IC/BPS biopsies before and after HOD, and between IC/BPS and control biopsies. Results Principal component analysis revealed complete separation between gene expression profiles from IC/BPS and control samples ( q ≤ 0.05) and while IC/BPS samples before and after HOD showed no significant differences in expressed genes, 68 transcripts were found to be significantly different between IC/BPS and control samples ( q ≤ 0.05). Conclusions The bladder HOD procedure itself does not significantly change gene expression within the IC/BPS patient bladder, a finding that provides evidence to support the use of biopsies from non‐IC/BPS patients that have not undergone HOD as controls for gene expression studies.