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Collagen I and collagen III polymorphisms in women with pelvic organ prolapse
Author(s) -
Batista Nilce C.,
Bortolini Maria A. T.,
Silva Rebecca S. P.,
Teixeira Juliana B.,
Melo Natália C.,
Santos Renata G. M.,
Pepicelli Fernanda A. A.,
Castro Rodrigo A.
Publication year - 2020
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.24447
Subject(s) - medicine , odds ratio , confidence interval , single nucleotide polymorphism , genotype , diabetes mellitus , gynecology , logistic regression , gastroenterology , endocrinology , genetics , gene , biology
Aims Verify the presence of the single nucleotide polymorphisms rs1800012 of the collagen I (COL1A1) and rs1800255 of the collagen III (COL3A1) genes and their association with pelvic organ prolapse (POP) in Brazilian women and to determine risk factors for POP. Methods We assessed 826 postmenopausal women divided into POP (stages III and IV) and control groups (stages 0 and I) by examination and peripheral blood sample collection. DNA sequences of interest were analyzed by real‐time reverse‐transcriptase polymerase chain reaction. We used logistic regression analyses, recessive and codominance models of inheritance, and P < .05 for significance. Results Six‐hundred and thirty‐four postmenopausal women were included: 348 (54.8%) POP cases and 286 (45.1%) controls. The frequencies of GG, GA, and AA genotypes for COL1A1 were 69.12%, 20.24%, and 10.59% in POP group and 71.79%, 20%, and 8.21% in controls; GG, GT, and TT for COL3A1 were 37.54%, 59.53%, and 2.93% in POP group and 36.24%, 60.14%, and 3.62% in controls. There were no genotypic or allelic association with POP phenotype that link both SNPs rs1800012 and rs1800255 to increased risk of POP. Vaginal delivery (odds ratio [OR] = 13; 95% confidence interval [CI] [4.00‐47.08]), POP family history (OR = 3.1; 95% CI [1.49‐6.50]), diabetes mellitus (OR = 2.3; 95% CI [1.08‐5.21]), number of pregnancies (OR = 1.2; 95% CI [1.05‐1.36]), and age (OR = 1.1; 95% CI [1.09‐1.19]), were variables of increased risk for POP ( P < .05 for all). Conclusion Our study suggests lack of association between DNA polymorphisms rs1800012 of COL1A1 and rs1800255 of COL3A1 with advanced POP. Vaginal delivery, POP family history, diabetes mellitus, number of pregnancies, and age are risk factors for POP.