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Functional heterogeneity of PDGFRα (+) cells in spontaneously active urogenital tissues
Author(s) -
Hashitani Hikaru,
Mitsui Retsu,
Lang Richard
Publication year - 2020
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.24431
Subject(s) - purinergic receptor , stimulation , lamina propria , pathology , medicine , interstitial cell of cajal , microbiology and biotechnology , immunohistochemistry , biology , endocrinology , receptor , epithelium
Aims As PDGFRα (+) cells appear not to suppress the excitability of detrusor smooth muscle by generating SK3‐dependent hyperpolarising as proposed in the gastrointestinal tract, we further explored the functional roles of PDGFRα (+) cells in regulating the spontaneous activity of urogenital tissues. Methods Using PDGFRα‐eGFP mice, intracellular Ca 2+ signaling in PDGFRα (+) cells of the bladder lamina propria, renal pelvis, and seminal vesicle were visualized using Cal‐590 fluorescence. The distribution and SK3 expression of PDGFRα (+) cells were also examined by immunohistochemistry. Results In the bladder lamina propria, SK3 (−) PDGFRα (+) cells exhibited spontaneous Ca 2+ transients and responded to stimulation of P2Y1 purinoceptors with MRS2365 (100 nM) or adenosine diphosphate (ADP) (100 μM) by developing Ca 2+ transients. In the proximal renal pelvis, PDGFRα (+) cells were distributed in the mucosal, muscular and serosal layers but did not express SK3 immunoreactivity. PDGFRα (+) cells in the musculature resembling atypical smooth muscle cells generated spontaneous Ca 2+ transients that were partially suppressed upon P2Y1‐stimulation, while vigorously responding to human angiotensin II (100 nM). In the seminal vesicle, PDGFRα (+) cells in the musculature but not mucosa expressed SK3 immunoreactivity. In the mucosa, the P2Y1 stimulation evoked Ca 2+ transients in both PDGFRα (+) cells and PDGFRα (−) cells. Conclusion PDGFRα (+) cells in spontaneously active urogenital tissues display heterogeneity in terms of their SK3 expression and P2Y1‐induced Ca 2+ responses. Muscular PDGFRα (+) cells in the renal pelvis and mucosal PDGFRα (+) cells in the seminal vesicle may generate depolarizing signals to drive smooth muscle cells.